물리적 휴면타파 처리가 시베리아잎갈나무 (Larix sibirica Ledeb.) 종자 발아에 미치는 영향 및 근적외선 분광 기반 평가

에네비시 도치카, 정은주*

(강원대학교 산림환경시스템학과)

Effects of Physical Dormancy-Bresking Treatments on Seed Germination of Larix Sibirica Ledeb. Assessed by Near-Infrared Spectroscopy

Enebish Dorjpagma, Eun Ju Cheong*

(Department of Forest Environment System, Kangwon National University)

Abstract: Larix sibirica Ledeb. is the dominant coniferous tree species in Mongolia, covering approximately 7.3 million hectares and accounting for about 63% of the country's total forest area. Due to climate change, seed production has declined, natural regeneration has slowed, and the risk of population decline has increased. Therefore, identifying effective dormancy-breaking treatments to enhance seed germination is of critical importance. Seeds were collected in 2024 from forest stands within the natural distribution range of Siberian larch in northern Mongolia. The effects of physical dormancy-breaking treatments, including hot water (600C, 800C, 1000C), ultrasound soaking (40 kHz for 40, 60, 80 minutes), and sulfuric acid (50% H2SO4 for 10, 20, 30 minutes), were investigated. Germination percentage was evaluated, and differences among treatments were analyzed using one-way analysis of variance (ANOVA). In addition, stereo microscopy and near-infrared (NIR) spectroscopy were employed as non-destructive tools to evaluate surface and internal changes in seed structure induced by physical dormancy-breaking treatments. Ultrasound treatment for 60 and 80 min and sulfuric acid treatment for 20 min resulted in germination rates of approximately 83%, which were significantly higher than those of the hot water treatment and the untreated control (p < 0.001). Among these treatments, ultrasound soaking for 80 minutes produced the highest germination rate Near-infrared (NIR) spectroscopy showed a clear trend at 1450 nm, where lower reflectance was associated with higher germination. Sulfuric acid treatment for 10 and 20 min and ultrasound soaking for 80 min exhibited lower reflectance and higher germination, whereas ultrasound soaking for 40 and 60 min and hot water treatment showed higher reflectance and reduced germination. One-way ANOVA confirmed a significant effect of treatments on reflectance at 1450 nm (p < 0.001), indicating that NIR reflectance is a reliable indicator of germination performance. These results demonstrate that physical weakening of the seed coat effectively enhances germination of L. sibirica seeds.

Keywords: Larix sibirica; seed germination; physical scarification; climate change; NIR spectroscopy